Chuanyue Wu, PhD
Professor of Pathology
Dr. Wu, Professor of Pathology and Lombardi and Shinozuka Experimental Pathology Research Chair, is a member of the Section of Laboratory Medicine.
S-707B Scaife Hall
University of Pittsburgh
3550 Terrace Street
Pittsburgh, PA 15261
Office Telephone: 412-648-2350
Lab Telephone: (412) 624-2471
- PhD - University of Pittsburgh, 1990
Awards and Honors
- 2011 - Pitt Innovator Award, University of Pittsburgh
- 2010 - Pitt Innovator Award, University of Pittsburgh
- 1998 - V Foundation Scholar, V Foundation for Cancer Research
- 1996 - Edward Livingston Trudeau Scholar, American Lung Association
- 1996 - Parker B. Francis Fellow
- 1996 - Edward C. Kendall Alumni Award for Meritorious Research, Mayo Foundation
Cell-extracellular matrix adhesion is a fundamental process that regulates cell shape, proliferation and differentiation. Abnormalities in cell-matrix adhesion and extracellular matrix assembly are closely associated with the pathogenesis of a variety of human diseases. The goals of my research are:
- To elucidate the molecular mechanism underlying cell-extracellular matrix adhesion and signaling
- To determine the role of integrin-linked kinase signaling in the pathogenesis of diabetic nephropathy
To achieve these goals, we are employing a wide variety of molecular and cell biological techniques including molecular cloning, mutagenesis, gene transfer, protein expression, yeast two-hybrid and other protein-protein interaction assays, hybridoma production, cell culture, cell adhesion, cell migration, immunofluorescence microscopy, immunoprecipitation, Northern, Southern and Western blotting, cell proliferation, apoptosis, and immunohistochemistry in our research. We have demonstrated that a protein complex consisting of PINCH-1, integrin-linked kinase (ILK) and parvin is crucial for cell-matrix adhesion, migration and survival signaling. Alterations of the PINCH-1-ILK-parvin complex are involved in the pathogenesis of diabetic nephropathy. Furthermore, we have identified a critical role of kindlin-2 in regulation of integrin activation and cell-matrix adhesion. Our studies shed new light on the molecular basis underlying cell-matrix and cell-cell communications as well as their roles in the pathogenesis of human diseases including diabetic nephropathy and cancer.
NIH ResearchView Dr. Wu's NIH RePORT on nih.gov
Selected PublicationsView Dr. Wu's publications on PubMed
- Davidson B, Holth A, Nguyen MT, Trop&eactue; CG, and Wu C. Migfilin, α-parvin and β-parvin are differentially expressed in ovarian serous carcinoma effusions, primary tumors and solid metastases. Gynecol Oncol. 2013. 128(2):364-70. PMCID: PMC3552080.
- Gkretsi, V., Papanikolaou, V., Dubos, S., Papathanasiou, I., Giotopoulou, N., Valiakou, V., Wu, C., Malizos, K. N., and Tsezou, A. Migfilin’s elimination from osteoarthritic chondrocytes further promotes the osteoarthritic phenotype via beta-catenin upregulation. Biochem. Biophys. Res. Commun. 2013, 430(2):494-9. PMCID: PMC3764434.
- Gkretsi, V., Papanikolaou, V., Zacharia, LC., Athanassiou, E., Wu, C. and Tsezou, A. Mitogen-inducible gene-2 (Mig-2) and Migfilin expression is reduced in human breast cancer samples. Anticancer Res. 2013, 33(5):1977-81.
- Song, C., Zhu, S., Wu, C*. and Kang, J*. Histone deacetylase (HDAC) 10 suppresses cervical cancer metastasis through inhibition of matrix metalloproteinase (MMP) 2 and 9 expression. J. Biol. Chem. 2013 Sep 27;288(39):28021-33 (*co-correspondence authors). PMCID: PMC3784715.
- Teräväinen ,T. P., Myllymäki, S. M., Friedrichs, J., Strohmeyer, N., Moyano, J.V., Wu, C., Matlin, K. S., Muller, D. J., and Manninen, A. αV-integrins are required for mechanotransduction in MDCK epithelial cells. PLoS One 2013 Aug 19;8(8):e71485. PMCID: PMC3747215.
- Donthamsetty, S., Bhave, V.S., Mars, W.M., Bowen, W.C., Orr, A., Haynes, M, M., Wu, C., Michalopoulos, G.K. Role of PINCH and Its Partner Tumor Suppressor Rsu-1 in Regulating Liver Size and Tumorigenesis. PLoS One 2013 Sep 18;8(9):e74625. PMCID: PMC3776730.
- Cui X, Zhao Z, Liu D, Guo T, Li S, Hu J, Liu C, Yang L, Cao Y, Jiang J, Liang W, Liu W, Li S, Wang L, Wang L, Gu W, Wu C, Chen Y, Li F. Inactivation of miR-34a by aberrant CpG methylation in Kazakh patients with esophageal carcinoma. J Exp Clin Cancer Res. 2014 Feb 17;33(1):20. [Epub ahead of print]
- Qu H, Tu Y, Guan JL, Xiao G, Wu C. Kindlin-2 tyrosine phosphorylation and interaction with Src serve as a regulatable switch in the integrin outside-in signaling circuit. J Biol Chem. 2014 Nov 7;289(45):31001-13.
- Zhu K, Yi J, Xiao Y, Lai Y, Song P, Zheng W, Jiao H, Fan J, Wu C, Chen D, Zhou J, Xiao G. Impaired bone homeostasis in amyotrophic lateral sclerosis mice with muscle atrophy. J Biol Chem. 2015 Mar 27;290(13):8081-94.
- Liu Z, Zhan Y, Tu Y, Chen K, Liu Z, Wu C. PDZ and LIM domain protein 1(PDLIM1)/CLP36 promotes breast cancer cell migration, invasion and metastasis through interaction with α-actinin. Oncogene. 2015 Mar 5;34(10):1300-11.