William A. LaFramboise, PhD
Associate Professor of Pathology

Dr. LaFramboise is a member of tthe Division of Molecular Genomic Pathology, and the University of Pittsburgh Cancer Institute. He directs the Genomics Division of the Cancer Biomarker Facility.

Office Location:
Rm. WG02.11
UPMC Shadyside
5230 Centre Avenue
Pittsburgh, PA 15213
Contact Information:
Office Telephone: 412-623-6160
Genomics Lab: 412-623-1497
Sequencing Lab: 412-864-7515
Email: laframboisewa@upmc.edu

Dr. LaFramboise works at the interface of translational research providing technical capabilities to meld basic research and technologies with clinical processes and discoveries in order to impact the diagnosis and treatment of diseases. Cellular and molecular techniques utilized in his laboratories are collaboratively provided to clinical and basic researchers in order to identify and manipulate underlying disease mechanisms.

Research Interests

High Throughput Genomic/Proteomics and Sequencing

Interrogation of genome-wide DNA, mRNA and microRNA profiles in cancer using 1) microarrays, 2) PCR, and 3) Next-Generation and Targeted Deep Sequencing of human specimens using (SOLiD) and single molecule sequencing detection (Ion Torrent).

Stem Cells and Tumor Stem Cells

Perform in vitro selection of stem cells intrinsic to normal tissues and tumors - media conditions and substrates established for culture of human embryonic stem cell lines select, isolate and amplify colony-forming cells from normal vs. tumor biopsy specimens.

Diagnosis of Coronary Artery Disease

Multiplex proteomics immunoassays of serum samples distinguish patients with coronary artery disease (CAD) requiring clinical intervention. A 5 protein signature and algorithm was developed to classify patients in need of therapy with 95% positive predictive value.

Clinical Genomics Facility

Development of novel assays to interrogate complex targets from difficult specimens such as stem cells, rare heterogenous tumors & FFPE samples. The facility has an internal normal DNA and SNP reference database for utilization in all SNP and sequencing studies.

Awards and Honors

University of Pittsburgh Innovator Award, 2008 - 2011

Recent Peer-Reviewed Publications

View Dr. LaFramboise's publications on PubMed

LaFramboise WA, Kelly LA, Petrosko P, Krill-Burger JM, Sciulli CM, BS1, Lyons MA, Chandran UR, Dhir R, Lomakin A, Masterson RV, Marroquin OC, Mulukutla SR, McNamara DM. Serum Proteomic Profiles Detect Coronary Artery Disease in Symptomatic Patients Referred for Coronary Angiography. BMC Medicine. 2012 Dec 5; 10 (1): 157.

Gullapalli R, Petrosko P , Lyons MA, Becich M, Dhir R, LaFramboise WA. Clinical Integration of Next Generation Sequencing Technology, Clinics in Laboratory Medicine, 2012 Dec; 32(4): 585-599.

Bonneh-Barkay D, Wang G, LaFramboise WA, Wiley CA, Bissel SJ Exacerbation of Experimental Autoimmune Encephalomyelitis in the Absence of Breast Regression Protein 39/Chitinase 3-Like 1. J Neuropathol Exp Neurol. 2012 Oct 4. E-pub ahead of print.

Satish L, LaFramboise WA, Johnson S, Vi L, Njarlangattil A, Raykha C, Krill-Burger JM, Gallo PH, O'Gorman DB, Gan BS, Baratz ME, Ehrlich GD, Kathju S. Fibroblasts from phenotypically normal palmar fascia exhibit molecular profiles highly similar to fibroblasts from active disease in Dupuytren's Contracture. BMC Med Genomics. 2012 May: (4): 5:15.

Yu YP, Song C, Tseng G, Ren BG, LaFramboise W, Michalopoulos G, Nelson J, Luo JH. Genome abnormalities precede prostate cancer and predict clinical relapse. Am J Pathol. 2012 Jun: 180(6):2240-2248.

Krill-Burger JM, Lyons MA, Kelly LA, Sciulli CM, Petrosko P, Chandran UR, Kubal MD, Bastacky SI, Parwani AV, Dhir R, LaFramboise WA. Renal Cell Neoplasms Contain Shared Tumor Type-Specific Copy Number Variations. Am J Pathol. 2012 Jun;180 (6):2427-39.

Manohar R, Komori J, Guzik L, Stolz DB, Chandran UR, LaFramboise WA, Lagasse E. Identification and expansion of a unique stem cell population from adult mouse gallbladder. Hepatology. 2011 Jul 25. doi: 10.1002/hep.24568.

Lisovich A, Chandran UR, Lyons-Weiler MA, LaFramboise WA, Brown AR, Jakacki RI, Pollack IF, Sobol RW. A novel SNP analysis method to detect copy number alterations with an unbiased reference signal directly from tumor samples. BMC Med Genomics. 2011 Jan 26;4(1):14.

Pollack IF, Hamilton RL, Sobol RW, Nikiforova MN, Lyons-Weiler MA, LaFramboise WA, Burger PC, Brat DJ, Rosenblum MK, Holmes EJ, Zhou T, Jakacki RI; Children's Oncology Group. IDH1 mutations are common in malignant gliomas arising in adolescents: a report from the Children's Oncology Group. Childs Nerv Syst. 2011 Jan;27(1):87-94.