Samuel A. Yousem, MD
E. Leon Barnes Professor of Anatomic Pathology

Dr. Yousem Dr. Yousem is the Vice Chairman of Anatomic Pathology Services at UPMC, Director of the Division of Anatomic Pathology, and Co-Director of the Community Pathology Division.

Office Location:
Department of Pathology A610
UPMC Presbyterian
200 Lothrop Street
Pittsburgh, PA 15213-2582
Contact Information:
Office Telephone: 412-647-6193
Fax: 412-647-3399


  • BS - 1977, Duke University
  • MD - 1981, University of Maryland School of Medicine

Clinical Expertise

Dr. Yousem is a general surgical pathologist who has devoted the last ten years of his practice to focus on thoracic pathology. This expertise extends from inflammatory to neoplastic thoracic pathology. Perhaps his greatest impact has been in the area of pulmonary allograft pathology where Dr. Yousem, working at Stanford University and The University of Pittsburgh defined the histopathology of pulmonary allograft rejection and its variations and has been responsible for the three major revisions of international standards creating a working formulation for the standardization of nomenclature in the diagnosis of lung rejection. This effort most recently has focused on defining criteria for antibody mediated rejection in the lung allograft. At the same time Dr. Yousem has a great interest in chronic fibrosing interstitial lung disease, and has written extensively on the pathology of usual interstitial pneumonia, nonspecific interstitial pneumonia, respiratory bronchiolitis, smoking induced interstitial fibrosis, and pulmonary vasculitis.

In recent years, working with his colleague Sanja Dacic, MD PhD, his academic focus has been on the specific molecular drivers of pulmonary neoplasia, their association with specific histopathologies, and the prediction of biologic behavior based on molecular and histopathologic alterations. This interest has extended to a wide variety of unusual pulmonary malignancies, where the molecular pathology has in large part been defined by our molecular thoracic group, including studies of bronchioalveolar carcinoma/adenocarcinoma in situ, atypical adenomatous hyperplasia, sclerosing hemangioma of lung, chemodectomas, Langerhans cell histiocytosis, adenosquamous carcinoma and micropapillary adenocarcinoma. Dr. Yousem leads one of the most academically productive and internationally recognized sections in thoracic pathology in the world.

Research Interests

As indicated above, Dr. Yousem, has focused his energies on three areas of pathology; lung transplantation pathology, interstitial lung disease and the molecular alterations in pulmonary neoplasia. In each of these areas he is certainly regarded as one of the world leaders in correlating special studies including immunohistochemistry, fluorescence in situ hybridization, and molecular techniques with histopathology in an attempt to expand the differential diagnosis, prognostic importance, and pathobiology of thoracic inflammatory and neoplastic disease.


Anatomic Pathology: Cytopathology


Thoracic Pathology

Awards and Honors

Leon Barnes Professor of Surgical Pathology, 2012 - endowed chair

Selected Publications

View Dr. Yousem's publications on PubMed

Striebel JM, Dacic S, Yousem SA. "Gross Cystic Disease Fluid Protein - (GCDFP-15) Expression in Primary Lung Adenocarcinoma". Am J Surg Pathol 32:426-432, 2008.

Stewart S, Fishbein MC, Snell, GI, Berry GJ, Boehler A, Burke MM, Glanville A, Gould K, Magro C, Marboe CC, McNeil KD, Reed EF, Reinsmoen NL, Scott JP, Studer SM, Tazelaar HD, Wallwork JL, Westall G, Zamora MR, Zeevi A, Yousem SA. Revision of the 1996 working formation for the standardization of nomenclature in the diagnosis of lung rejection. J of Heart and Lung Transpl 26:1229-42, 2007.

Yousem SA, Nikiforova M, Nikiforov Y. The histopathology of BRAF-V600E mutated lung adenocarcinoma. Am J Surg Pathol 32:1317-21, 2008.

Li S, Salgar SK, Kurimoto Y, Yousem S, Pham SM. Mixed chimerism achieved by a nonlethal conditioning regimen induces donor-specific tolerance to lung allografts. J Surg R 146:289-97, 2008.

Chen Z-H, Hong P-K, Zhang Y, Lee SJ, Feghali-Bostwick C, Stolz DB, Dhir, R, Landreneau RJ, Schuchert MJ, Yousem SA, Nakahira K, Pilewski JM, Lee J, Sciurba F, Ryter SW, Choi AMK. EGR-1 regulates autophagy in cigarette smoke-induced i njury and chronic obstructive pulmonary disease. PLos ONE 3:3316, 2008.

Girnita AL, Lee MT, McCurry KR, Baldwin WM, Yousem SA, Detrick B, Pilewski J, Toyoda Y, Jelinek L, Lomago J, Zaldonis D, Spichty KJ, Zeevi A. Anti-human leukocyte antigen antibodies, vascular C4d deposition and increased soluble c4d in broncho-alveolar lavage of lung allografts. Transpl 86:342-7, 2008.

McHayleh W, Morcos JP, Wu T, Shapiro WR, Yousem SA, Appleman L, Friedland DM. Renal Cell Carcinoma from a Transplanted Allograft: Two Case Reports and a Review of the Literature. Clin Genitourin Cancer 6(1): 53-5, 2008.

Yousem SA. Peripheral squamous cell carcinoma of lung: patterns of growth with particular focus on airspace filling and its clinical relevance. Human Pathol 40:861-867, 2009.

Konishi K, Gibson KF, Lindell KO, Richards TJ, Zhang Y, Dhir R, Bisceglia M, Gilbert S, Yousem SA, Song J-W, Kim DS, Kaminski N. Gene expression profiles of acute exacerbations of idiopathic pulmonary fibrosis. Am J Respir Crit Care Med 2009 180:167-175, 2009.

Yousem SA. The surgical pathology of pulmonary infarcts, diagnostic confusion with granulomatous disease, vasculitis, and neoplasia. Mod Pathol 22:679-685, 2009.

Achcar RD, Nikiforova MN, Dacic S, Nicholson AG, Yousem SA. Mammalian mastermind like 2 11q21 gene rearrangement in bronchopulmonary mucoepidermoid carcinoma. Hum Pathol 40:854-860, 2009.

Achcar RD, Nikiforova MN, Dacic S, Yousem SA Micropapillary Lung Adenocarcinoma: EGFR, KRAS, and BRAF Mutational Profile. Am J Clin Pathol 131:694-700, 2009.

Di Giuseppe M, Gambelli F, Hoyle GW, Lungarella G, Studer SM, Richards T, Yousem S, McCurry K, Dauber J, Kaminski N, Leikauf G, Ortiz LA. Systemic Inhibition of NF-κB Activation Protects from Silicosis. PLoS ONE 4(5): e5689. doi:10.1371/journal.pone.0005689, 2009.

Richards TJ, Eggebeen A, Gibson K, Yousem S, Fuhrman C, Gochuico BR, Fertig N, Oddis CV, Kaminski N, Rosas IO, Ascherman DP. Characterization and peripheral blood biomarker assessment of anti-Jo-1 antibody-positive interstitial lung disease. Arthritis Rheum 60:2183-92, 2009.

Dacic S, Shuai Y, Yousem S, Ohori P, Nikiforova M. Clinicopathological predictors of EGFR/KRAS mutational status in primary lung adenocarcinomas. Mod Pathol 23:159-168, 2009.

Vadnerkar A, Clancy CJ, Toyoda Y, Yousem SA, Celik U, Nguyen ML, Pilewski J, Kwak EJ, Nguyen MH. Impact of fungal infections in explanted lungs on outcomes of lung transplantation. (Transplantation 89:253-260, 2010).

Hwang DM, Yousem S. Approach to a lung transplant biopsy. J Clin Pathol 63:38-46, 2010.

Fitches AC, Yousem S, Cieply K, Stebbings S, Highton J, Hung NA. PCR detectable Y chromosome-specific DNA but no intact Y chromosome-bearing cells in polymyositis biopsies of two women with male offspring. Pathology 42:160—4, 2010.

Pandit KV, Corcoran D, Yousef H, Yarlagadda M, Handley D, Konishi K, Gibson K, Tzouvelekis A, Yousem S, Singh M, Richards T, Selman M, Pardo A, Ben-Yehudah A, Bouros D, Eickelberg O, Ray P, Benos PV, Kaminski N. Inhibition and Role of LET-7 in Idiopathic Pulmonary Fibrosis. Am J Respir Crit Care Med 182(2): 220-229, 2010.

Yousem, Samuel A, Gibson, Kevin, Kaminski, Naftali, Oddis, Chester V, Ascherman, Dana P. The Pulmonary Histopathologic Manifestations of the anti-Jo-1 tRNA Synthetase Syndrome. Mod Pathol 23(6):874-880, 2010.

Schuchert MJ, Schumacher L, Kilic A, Close JM, Landreneau JR, Pennathur A, Awais O, Yousem SA, Wilson DO, Luketich JD, Landreneau RJ. Impact of angiolymphatic invasion on outcomes following resection of stage I non-small cell lung cancer. Ann of Thor Surg 91(4): 1059-65, 2011.

Owens SR, W. L., Kelly SM, Piccoli AL, Lassige K, Yousem SA, Dhir R, Parwani AV. "Initial experience with a novel pre-sign-out quality assurance tool for review of random surgical pathology diagnoses in a subspecialty-based university practice." Am J Surg Pathol 34(9): 1319-1323, 2010.

Dacic S, Yousem S. "Molecular testing in lung carcinoma: Quo vadis?" Am J Clin Pathol 134(1): 7-9, 2010.

Owens SR, Dhir R., Yousem SA, Kelly SM, Piccoli A, Wiehagen L, Lassige K, Parwani AV "The development and testing of a laboratory information system-driven tool for pre-sign-out quality assurance of random surgical pathology reports." Am J Clin Pathol 133(6): 829-831, 2010.

Yousem SA, Faber C. "Histopathology of aspiration pneumonia not associated with food or other particulate matter: a clinicopathologic study of 10 cases diagnosed on biopsy." Am J Surg Pathol 35(3): 426-431, 2011.

Marchevsky A, Marx A, Strobel P, Suster S, Vernuta F, Marino M, Yousem S, Zakowski M. Policies and Reporting Guidelines for Small Biopsy Specimens of Mediastinal Masses. J Thor Onc 6:7-13, 2011.

Tochigi N, Dacic S, Nikiforova M, Cieply KM, Yousem SA. Adenosquamous carcinoma of the lung: a microdissection stud of KRAS and EGFR mutational and amplification status in a western patient population. Am J Clin Pathol 135(5): 783-9, 2011.

Myers JL, Yousem SA, DeYoung BR, Cibull ML; Council of the Association of Directors of Anatomic and surgical Pathology. Matching residents to pathology fellowships: the road less traveled? Am J Clin Pathol 135(3): 335-7, 2011.

Horne ZD, Jack R, Gray ZT, Siegfried JM, Wilson DO, Yousem SA, Nason KS, Landreneau RJ, Luketich JD, Schuchert MJ. Increased levels of tumor-infiltrating lymphocytes are associated with improved recurrence-free survival in Stage 1A non-small-cell lung cancer. J Surg Res (E-pub) April 22, 2011.

Owens SR, Wiehagen L, Simmons C, Sikorova A, Stewart W, Kelly S, Nestler R, Yousem SA. Numerical fidelity of endoscopic biopsy fragments in the processing sequence of a university surgical pathology laboratory. Arch pathol Lab Med 135:1561-4, 2011.

Gochuico BR, Yu G, Konishi K, Handley D, Richards T, Dhir R, Yousem S, Ren P, MacDonald SD, Wu HP, Stylianou M, Nathan SD, Billings E, Pacheco-Rodriguez G, Moss J, Kaminski N, Rosas IO. A Critical Role for Alveolar Macrophage Syndecan-2 in the Clinical Progression of Idiopathic Pulmonary Fibrosis. (In press).

Ortiz L, Fattman C, Sala E, Torres G, Njah J, Snyder J, Lotoche J, Kaminsky N, Yousem S, Leikauf G, Stripp B. Clara cell secretory protein (CCSP) regulates the innate immune response in silicosis. (Submitted to J of Clin Invest)

Schneider F, Hwang DM, Gibson K, Yousem SA. Non-Specific Interstitial Pneumonia (NSIP): A Study of Six Patients with Progressive Disease. Am J Surg Pathol 36:89-93, 2012.

Yousem SA. The Role of Molecular Studies in the Diagnosis of Lung Adenocarcinoma. Modern Pathol 25:S11-S17, 2012.

Yu G, Kovkarova-Naumovski E, Parwani A, Kass D, Ruiz V, Lopez-Otín C, Rosas IO, Jara P, Cabrera S, Yousem SA, Richards TJ, Chensny LJ, Selman M, Kaminski N, Pardo A. MMP19 is a key regulator of Lung Fibrosis in mice and humans.

Zappala CJ, Desai SR, Nicholson AG, Colby TV, Yousem S, Maher TM, Renzoni EA, duBois RM, Hansell DM, Wells, AU. Bronchioalveolar lavage cellularity in hypertensensitivity pneumonitis identifies patients with survival similar to IPF. (Submitted)

Yousem SA, Zeevi, A. The histopathology of lung allograft dysfunction associated with the development of donor specific HLA alloantibodies. (Accepted by AJSP).